Overview

  • Product nameAnti-MARVELD2 antibody
    See all MARVELD2 primary antibodies
  • Description
    Rabbit polyclonal to MARVELD2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide corresponding to a region between residue 90 and 140 of human MARVELD2 (NP_001033692.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTight junctions (TJ) prevent leakage of solutes through the paracellular pathway of epithelial cells. MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts. It plays a role in the formation of the epithelial barriers.
    • Cellular localizationCell membrane; Multi-pass membrane protein. Cell junction, tight junction. Note=Found at tricellular contacts.
    • Database links
    • Alternative names
      • DFNB49 antibody
      • FLJ30532 antibody
      • MARVD2 antibody
      • MARVEL (membrane associating) domain containing 2 antibody
      • MARVEL domain containing 2 antibody
      • MARVEL domain containing protein 2 antibody
      • MARVELD 2 antibody
      • MARVELD2 antibody
      • MRVLDC2 antibody
      • Tric antibody
      • Tricellulin antibody
      see all

    Anti-MARVELD2 antibody images

    • All lanes : Anti-MARVELD2 antibody (ab80421) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 64 kDa
      Observed band size : 64 kDa


      Exposure time : 10 minutes
    • Detection of human MARVELD2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80421 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80421 was used. Lane 2 represents control IgG IP.
      Detection: chemiluminescence with an exposure time of 10 seconds.

    References for Anti-MARVELD2 antibody (ab80421)

    This product has been referenced in:
    • Masuda R  et al. Negative regulation of the tight junction protein tricellulin by snail-induced epithelial-mesenchymal transition in gastric carcinoma cells. Pathobiology 77:106-13 (2010). Read more (PubMed: 20332670) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab80421.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"