Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556)

Overview

  • Product nameAnti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description
    Rabbit polyclonal to MASH1/Achaete-scute homolog 1
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MASH1/Achaete-scute homolog 1 aa 166-196 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P50553

  • Positive control
    • WB: Mouse lung tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 24947819
ICC/IF 1/500.
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • FunctionMay play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Anti-MASH1/Achaete-scute homolog 1 antibody images

  • Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556) at 1/500 dilution + Mouse lung tissue lysate

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 15 kDa,28 kDa,45 kDa (possible dimer),55 kDa (possible dimer). We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of mouse adult NSCs derived from SVZ, staining TTF1 with ab38556.

    Cells were fixed with Zamboni's fixative, permeabilized with 0.5% Triton X-100 and blocked with 3% goat serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/500 in 3% goat serum) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556)

This product has been referenced in:
  • Sandstrom RS  et al. Epigenetic Regulation by Chromatin Activation Mark H3K4me3 in Primate Progenitor Cells within Adult Neurogenic Niche. Sci Rep 4:5371 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24947819) »
  • Haskins WE  et al. Molecular Characteristics in MRI-Classified Group 1 Glioblastoma Multiforme. Front Oncol 3:182 (2013). IHC-Fr ; Human . Read more (PubMed: 23875172) »

See all 3 Publications for this product

Product Wall

Please thank your customer for getting back to us. I am very happy to hear that the staining with Anti-MASH1/Achaete-scute homolog 1 antibody gives a good result.

The image shows the nuclear localization of the protein very nicely.

...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cultured Cells (adult NSC derived from SVZ (neurosphere))
Specification adult NSC derived from SVZ (neurosphere)
Fixative Zamboni's Fixative
Permeabilization Yes - 0.5% TritonX-100 for 10min on ice
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Michael Weible

Verified customer

Submitted Nov 08 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 03 2012

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 03 2012

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Gracias por tu respuesta.

Ya he realizado el nuevo pedido completamente gratuito del anticuerpo los anticuerpos ab108937 y ab135273. Recibiréis una confirmación automática del mismo con más detalles.

Si n...

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Muchas gracias por tu respuesta.

He comentado el protocolo seguido en IHC-P con mis compañeros del laboratorio, para tratar de entender el problema ocurrido con Anti-MASH1/Achaete-scute homolog 1 antibody (ab38556). Sin embargo me conf...

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Gracias por contactarnos de nuevo.

Lamento mucho que sigas teniendo problemas con dos de los anticuerpos enviados, y te agradezco que nos hayas enviado las imágenes.

En relación al MASH1, me temo que no puedo ofrecerte...

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Expiration date: 2012-11-09


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