Anti-MASH1/Achaete-scute homolog 1 antibody (ab76987)

Overview

  • Product nameAnti-MASH1/Achaete-scute homolog 1 antibody
    See all MASH1/Achaete-scute homolog 1 primary antibodies
  • Description
    Mouse monoclonal to MASH1/Achaete-scute homolog 1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment: GFATLREHVP NGAANKKMSK VETLRSAVEY IRALQQLLDE HDAVSAAFQA GVLSPTISPN YSNDLNSMAG SPVSSYSSDE GSYDPLSPEE QELLDFTNWF, corresponding to amino acids 137-237 of Human Achaete-scute homolog 1 (NP_004307) with tag.

  • Positive control
    • Achaete-scute homolog 1 transfected 293T cell lysate. Recombinant tagged human Achaete-scute homolog 1 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab76987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Achaete-scute homolog 1 is approximately 1ng/ml if used as a capture antibody.

Target

  • FunctionMay play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Achaete scute complex homolog 1 antibody
    • Achaete scute complex homolog like 1 antibody
    • Achaete scute complex homologue 1 antibody
    • Achaete scute complex homologue like 1 antibody
    • Achaete scute complex like 1 antibody
    • Achaete scute protein antibody
    • Achaete-scute homolog 1 antibody
    • Ascl 1 antibody
    • ascl1 antibody
    • ASCL1_HUMAN antibody
    • Ash 1 antibody
    • ASH-1 antibody
    • Ash1 antibody
    • bHLHa46 antibody
    • Class A basic helix-loop-helix protein 46 antibody
    • Hash 1 antibody
    • HASH1 antibody
    • Mammalian achaete scute homolog 1 antibody
    • Mammalian achaete scute homologue 1 antibody
    • Mash 1 antibody
    • Mash1 antibody
    see all

Anti-MASH1/Achaete-scute homolog 1 antibody images

  • Anti-MASH1/Achaete-scute homolog 1 antibody (ab76987) at 5 µg/ml + Recombinant tagged human Achaete-scute homolog 1 fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab76987) at 5 µg/ml

    Lane 1 : Achaete-scute homolog 1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

References for Anti-MASH1/Achaete-scute homolog 1 antibody (ab76987)

ab76987 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"