Overview

  • Product name
  • Description
    Rabbit polyclonal to MASP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MASP1.

  • Positive control
    • Recombinant Human MASP3, Catalytic domain (aa 450-721).
  • General notes

    This product was previously named "Anti-MASP3 antibody".

    MASP1 is an isoform of MASP3. As MASP1 is more commonly used in scientific literature, this product is named after it. However, this antibody specifically detect MASP3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 45 kDa.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Complement factor MASP-3 antibody
      • Complement factor MASP3 antibody
      • Complement-activating component of Ra-reactive factor antibody
      • CRARF antibody
      • Mannose binding lectin associated serine protease 1 antibody
      • Mannose binding protein associated serine protease antibody
      • Mannose-binding lectin-associated serine protease 1 antibody
      • Mannose-binding lectin-associated serine protease-1 antibody
      • MASP antibody
      • PRSS 5 antibody
      • Ra-reactive factor serine protease p100 antibody
      • RaRF antibody
      • Serine protease 5 antibody
      see all

    Anti-MASP1 antibody images

    • All lanes : Anti-MASP1 antibody (ab65900) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : 80ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 3 : 40ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 4 : 20ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 5 : 10ng Recombinant Human MASP1 Catalytic Domain (aa450-721)
      Lane 6 : 5ng Recombinant Human MASP1 Catalytic Domain (aa450-721)


      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

      Recombinant Human MASP1 Catalytic Domain (aa450-721) loaded at 4.0, 2.0, 1.0, 0.5 and 0.25ng/µl.

    References for Anti-MASP1 antibody (ab65900)

    ab65900 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    5 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Abreviews
    Application
    Western blot
    Sample
    Human Serum (human and murein serum)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    8 µg
    Specification
    human and murein serum
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up