Overview

  • Product nameAnti-MASP1 antibody
    See all MASP1 primary antibodies
  • Description
    Rabbit polyclonal to MASP1
  • Specificityab72659 detects endogenous levels of fragment of activated MASP1 resulting from cleavage adjacent to Arg448.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human MASP1.

  • Positive control
    • Extracts from A549 cells treated with etoposide (25uM, 24hours).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
ELISA 1/10000.

Target

  • RelevanceMASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Complement factor MASP-3 antibody
    • Complement factor MASP3 antibody
    • Complement-activating component of Ra-reactive factor antibody
    • CRARF antibody
    • Mannose binding lectin associated serine protease 1 antibody
    • Mannose binding protein associated serine protease antibody
    • Mannose-binding lectin-associated serine protease 1 antibody
    • Mannose-binding lectin-associated serine protease-1 antibody
    • MASP antibody
    • PRSS 5 antibody
    • Ra-reactive factor serine protease p100 antibody
    • RaRF antibody
    • Serine protease 5 antibody
    see all

Anti-MASP1 antibody images

  • All lanes : Anti-MASP1 antibody (ab72659) at 1/500 dilution

    Lane 1 : Extracts from A549 cells, treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from A549 cells, treated with etoposide (25uM, 24hours) with Cl-peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-MASP1 antibody (ab72659)

ab72659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"