Overview

  • Product name
    Anti-MASPIN antibody
    See all MASPIN primary antibodies
  • Description
    Rabbit polyclonal to MASPIN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human MASPIN aa 1-231. Isoform 2 (BC020713).
    Sequence:

    MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDT ANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNL STEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILAD NSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRS PIIDVKNDRDRVGHKSIPMRNLRARPAKCLS


    Database link: P36952-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Mouse esophagus tissue and Human cervical cancer tissue lysates; Human esophagus cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab182785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/300.

Target

  • Function
    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity
    Normal mammary epithelial cells.
  • Sequence similarities
    Belongs to the serpin family. Ov-serpin subfamily.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • protease inhibitor 5 (maspin) antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpin B5 antibody
    • serpin family B member 5 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpinb5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Images

  • Western blot - Anti-MASPIN antibody (ab182785)
    Western blot - Anti-MASPIN antibody (ab182785)
    All lanes : Anti-MASPIN antibody (ab182785) at 1/1000 dilution

    Lane 1 : Mouse esophagus tissue lysate
    Lane 2 : Human cervical cancer tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG at 1/10000 dilution

    Predicted band size: 25 kDa


    Exposure time: 13 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (ab182785)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASPIN antibody (ab182785)

    Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ab182785 at dilution 1/60,on the right is trested with the fusion protein

References

ab182785 has not yet been referenced specifically in any publications.

Publishing research using ab182785? Please let us know so that we can cite the reference in this datasheet.

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