Overview

  • Product nameAnti-MASPIN antibody
    See all MASPIN primary antibodies
  • Description
    Rabbit polyclonal to MASPIN
  • SpecificityThis antibody detects endogenous levels of total MASPIN protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from an internal region of human MASPIN

  • Positive control
    • Extracts from Hela cells

Properties

Applications

Our Abpromise guarantee covers the use of ab65136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

  • FunctionTumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificityNormal mammary epithelial cells.
  • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • protease inhibitor 5 (maspin) antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpin B5 antibody
    • serpin family B member 5 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpinb5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Anti-MASPIN antibody images

  • All lanes : Anti-MASPIN antibody (ab65136) at 1/500 dilution

    Lane 1 : Extracts from Hela cells
    Lane 2 : Extracts from Hela cells, plus immunising peptide


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab65136 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MASPIN antibody (ab65136)

This product has been referenced in:
  • Todd AG  et al. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. J Mol Biol 401:681-9 (2010). Read more (PubMed: 20620147) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"