Overview

  • Product name
  • Description
    Rabbit polyclonal to MASPIN
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: TKECPFRLNK TDTKPVQMMN MEATFCMGNI DSINCKIIEL PFQNKHLSMF, corresponding to internal sequence amino acids 180-229 of Human MASPIN

  • Positive control
    • WB: HepG2 cell lysate IHC-P: Human skin tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab66513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.625 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity
    Normal mammary epithelial cells.
  • Sequence similarities
    Belongs to the serpin family. Ov-serpin subfamily.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • protease inhibitor 5 (maspin) antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpin B5 antibody
    • serpin family B member 5 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpinb5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Images

  • Anti-MASPIN antibody (ab66513) at 0.625 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling MASPIN with ab66513 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

References

ab66513 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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