Anti-MASPIN antibody [AT2N6] (ab95451)

Overview

  • Product nameAnti-MASPIN antibody [AT2N6]
    See all MASPIN primary antibodies
  • Description
    Mouse monoclonal [AT2N6] to MASPIN
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human MASPIN purified from E. coli

  • Positive control
    • HeLa cell lysate, HeLa cells
  • General notesab95451 derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant Human MASPIN.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesProtein A/G affinity chromatography
  • ClonalityMonoclonal
  • Clone numberAT2N6
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab95451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/500.

Target

  • FunctionTumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificityNormal mammary epithelial cells.
  • Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • protease inhibitor 5 (maspin) antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpin B5 antibody
    • serpin family B member 5 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpinb5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Anti-MASPIN antibody [AT2N6] images

  • Anti-MASPIN antibody [AT2N6] (ab95451) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat anti-mouse secondary conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 42 kDa
  • Immunofluorescence of Human HeLa cells stained with PI (Red) and ab95451 (1:500) with Alexa 488 (Green).
  • ICC/IF analysis of Maspin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Maspin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

References for Anti-MASPIN antibody [AT2N6] (ab95451)

ab95451 has not yet been referenced specifically in any publications.

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Thank you for contacting us. We have indeed in our catalog 5 unconjugated anti-interferon alpha 1 antibodies tested and guaranteed to work in Western Blot and Human samples. Please note that, unfortunately, import restrictions make the ...

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