Anti-Mast Cell Chymase antibody (ab123510)

Overview

  • Product nameAnti-Mast Cell Chymase antibody
    See all Mast Cell Chymase primary antibodies
  • Description
    Goat polyclonal to Mast Cell Chymase
  • Tested applicationsSuitable for: IHC, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Mast Cell Chymase aa 96-110 (internal sequence).
    Sequence:

    C-QFRHPKYNTSTLHHD


    Database link: P23946

  • Positive control
    • HEK293 lysate transiently expressing full-length Human Mast Cell Chymase

Properties

Applications

Our Abpromise guarantee covers the use of ab123510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC Use a concentration of 2 - 4 µg/ml.
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:128,000.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 27 kDa.

Target

  • FunctionMajor secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
  • Tissue specificityMast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
  • Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted. Cytoplasmic granule. Mast cell granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-chymase antibody
    • Chymase 1 antibody
    • Chymase 1 mast cell antibody
    • chymase 1 preproprotein transcript E antibody
    • chymase 1 preproprotein transcript I antibody
    • Chymase antibody
    • Chymase, heart antibody
    • Chymase, mast cell antibody
    • CMA1 antibody
    • CMA1_HUMAN antibody
    • CYH antibody
    • CYM antibody
    • EC 3.4.21.39 antibody
    • Mast cell chymase 1 antibody
    • Mast cell protease 3 antibody
    • Mast cell protease 5 antibody
    • Mast cell protease I antibody
    • Mast cell protease III antibody
    • Mcp-5 antibody
    • MCP3P antibody
    • Mcpt5 antibody
    • MCT1 antibody
    • MGC119890 antibody
    • MGC119891 antibody
    • MMCP-5 antibody
    see all

Anti-Mast Cell Chymase antibody images

  • Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling Mast Cell Chymase with ab123510 at 2 ug/ml. Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The HRP-staining procedure was used for detection.

  • Lane 1 : Anti-Mast Cell Chymase antibody (ab123510) at 0.5 µg/ml
    Lane 2 : Anti-Mast Cell Chymase antibody (ab123510) at 1 mg/ml
    Lane 3 : Anti-tag antibody at 1/3000 dilution

    Lane 1 : HEK293 lysate over expressing Human CMA1 with a tag at 10 µg
    Lane 2 : Mock-transfected HEK293 lysate
    Lane 3 : HEK293 lysate over expressing Human CMA1 with a tag at 10 µg

    Developed using the ECL technique

    Predicted band size : 27 kDa

References for Anti-Mast Cell Chymase antibody (ab123510)

ab123510 has not yet been referenced specifically in any publications.

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The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

We unfortunately do not have any extra immu...

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