Anti-Mast Cell Chymase antibody [CC1] (ab2377)

Overview

  • Product nameAnti-Mast Cell Chymase antibody [CC1]
    See all Mast Cell Chymase primary antibodies
  • Description
    Mouse monoclonal [CC1] to Mast Cell Chymase
  • SpecificityAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat, Human, Pig
  • Immunogen

    Purified human skin Chymase.

  • Positive control
    • Tonsil. IHC-P: FFPE human tonsil tissue sections.
  • General notes


    Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

Properties

Applications

Our Abpromise guarantee covers the use of ab2377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. 1/100-1/200 in an ABC method.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionMajor secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
    • Tissue specificityMast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
    • Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localizationSecreted. Cytoplasmic granule. Mast cell granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-chymase antibody
      • Chymase 1 antibody
      • Chymase 1 mast cell antibody
      • chymase 1 preproprotein transcript E antibody
      • chymase 1 preproprotein transcript I antibody
      • Chymase antibody
      • Chymase, heart antibody
      • Chymase, mast cell antibody
      • CMA1 antibody
      • CMA1_HUMAN antibody
      • CYH antibody
      • CYM antibody
      • EC 3.4.21.39 antibody
      • Mast cell chymase 1 antibody
      • Mast cell protease 3 antibody
      • Mast cell protease 5 antibody
      • Mast cell protease I antibody
      • Mast cell protease III antibody
      • Mcp-5 antibody
      • MCP3P antibody
      • Mcpt5 antibody
      • MCT1 antibody
      • MGC119890 antibody
      • MGC119891 antibody
      • MMCP-5 antibody
      see all

    Anti-Mast Cell Chymase antibody [CC1] images

    • IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml

      Lane 1 : Recombinant Human Chymase Protein
      Lane 2 : Recombinant Human Mast Cell Tryptase protein (ab134529)

      Lysates/proteins at 0.1 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 27 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

       

       

      Ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.

    References for Anti-Mast Cell Chymase antibody [CC1] (ab2377)

    This product has been referenced in:
    • Zheng J  et al. Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS One 9:e94732 (2014). ICC ; Mouse . Read more (PubMed: 24733352) »
    • Yadav A  et al. Altered immunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium. PLoS One 9:e98719 (2014). IHC-P ; Human . Read more (PubMed: 24874976) »

    See all 14 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
    Permeabilization No
    Specification skin
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 15 2016

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Human Cell (Mast cell)
    Specification Mast cell
    Permeabilization No
    Fixative BD Biosciences fixation/permeabilization solution
    Username

    Abcam user community

    Verified customer

    Submitted Aug 05 2014

    You are very welcome.

    Please let us know if there are ever any other ways in which Abcam might help you.

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Thank you for contacting us.

    The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

    We unfortunately do not have any extra immu...

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    Vielen Dank für Ihre Anfrage.

    Es freut mich sehr zu hören, dass ab2377 gute Ergebnisse liefert.

    Ich persönlich habe leider keine Erfahrung mit Sirius Red. Meine Kollegen allerdings haben Sirius Red schon verwendet. Al...

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    Vielen Dank für Ihre Anfrage.

    Die Abbildung von ab54176 wurde mit einer Gegenfärbung mit Eosin (alleine) gefärbt.

    Während die Abbildung von ab111239 eine Gegenfärbung mit Hematoxlin zeigt.

    Beide k&...

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    I am very pleased to hear you would like to accept our offer and test ab2377 with dog samples. This code will give you 1 free PRIMARY ANTIBODY before the e...

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    Thank you very much for your interest in ab2377. To our knowledge, ab2377 has not been tested in mouse and rat. Therefore, I can offer a discount off a future purchase if you buy ab2377 now, test it in mouse and rat and submit feedback to us in...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Kidney)
    Specification Kidney
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% H2O2
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Prof. Chung-Ming Chen

    Verified customer

    Submitted Jan 09 2009

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"