Anti-Mast Cell Tryptase antibody [AA1] (ab2378)

Overview

  • Product name
    Anti-Mast Cell Tryptase antibody [AA1]
    See all Mast Cell Tryptase primary antibodies
  • Description
    Mouse monoclonal [AA1] to Mast Cell Tryptase
  • Specificity
    This antibody reacts with mast cells distributed in skin, synovium, lung and heart. It does not bind with any other cell type. Recognises human mast cell tryptase, both alpha and beta isoforms
  • Tested applications
    Suitable for: ICC, WB, IHC-P, ICC/IF, IHC-R, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cat, Dog, Monkey
  • Immunogen

    Purified human lung Tryptase

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Lung; Tonsil; Skin.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab2378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 31 kDa).

We recommend using 3% milk as the blocking agent for Western blot.

IHC-P Use a concentration of 0.1 µg/ml.
ICC/IF 1/100.
IHC-R Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • Cellular localization
    Secreted. Note: released from the secretory granules upon mast cell activation.
  • Database links
  • Alternative names
    • alpha II antibody
    • Lung tryptase antibody
    • Mast cell alpha II tryptase antibody
    • Mast cell beta I tryptase antibody
    • Mast cell protease 7 antibody
    • Mast cell protease II antibody
    • MCP 7 antibody
    • Pituitary tryptase antibody
    • Skin tryptase antibody
    • TPS 1 antibody
    • TPS1 antibody
    • TPS2 antibody
    • TPSAB1 antibody
    • TPSAB1 protein antibody
    • TPSB1 antibody
    • Tryptase 1 antibody
    • Tryptase alpha 1 antibody
    • tryptase alpha I included antibody
    • Tryptase alpha II antibody
    • tryptase alpha II included antibody
    • tryptase alpha included antibody
    • tryptase alpha/beta 1 antibody
    • Tryptase beta 1 antibody
    • tryptase beta I included antibody
    • Tryptase I antibody
    • tryptase I included antibody
    • Tryptase III antibody
    • Tryptase skin antibody
    see all

Images

  • All lanes : Anti-Mast Cell Tryptase antibody [AA1] (ab2378) at 5 µg/ml

    Lane 1 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
    Lane 2 : Human tonsil normal tissue lysate - total protein (ab29615)
    Lane 3 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 35-37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Mast Cell Tryptase contains an 18-residue signal sequence and a 12-residue activation peptide. The protein also has two potential glycosylation sites (Swissprot data). These post-translational modifications might explain the banding pattern observed. Abcam used 5% milk in TBS-T as a blocking agent for this blot. We found that this blocking agent removed more non-specific bands than BSA.

  • ab2378 staining Mast Cell Tryptase in Human Tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/7500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). Tissue was fixed with acetone and inhibitors and embedded in GMA resin. Samples were incubated with primary antibody (1/2000 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Super block for 1 hour at 22°C. Samples were incubated with primary antibody (1/150 in PBS + 10% super block) for 14 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Mouse Lung (LPS inflammation) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% M.O.M Ig blocking for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab2378 staining formalin fixed paraffin-embedded human colon tissue sections cut at 1 micron.  The section was subjected to heat mediated antigen retrieval in Dako Target Retrieval Solution and permeabilized in Triton-X prior to blocking in 6% BSA for 2 hours at 24°C.  The primary anitbody was diluted 1/250 and incubated with the sample for 16 hours at 5°C.  The secondary antibody was ab7064 (diluted 1/2000) and the slide was counterstained with DAPI.

    See Abreview

  • ab2378 staining Mast Cell Tryptase in Human mast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with Saponin 0.1% in PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 14 hours at 4°C. A Texas Red®-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Niu R  et al. Inhibition of airway inflammation in a cockroach allergen model of asthma by agonists of miRNA-33b. Sci Rep 7:7409 (2017). Read more (PubMed: 28785038) »
  • Lee JY  et al. Repeated Water Avoidance Stress Alters Mucosal Mast Cell Counts, Interleukin-1ß Levels with Sex Differences in the Distal Colon of Wistar Rats. J Neurogastroenterol Motil 22:694-704 (2016). Read more (PubMed: 27466288) »

See all 22 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (nasal mucosa)
Permeabilization
No
Specification
nasal mucosa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

Submitted Jun 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Tissue (Colon)
Specification
Colon
Username

Abcam user community

Verified customer

Submitted May 04 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung Biopsy)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, ph=6
Permeabilization
No
Specification
Lung Biopsy
Fixative
Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Apr 28 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mature (8weeks) and immature BMMCs)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
2 µg
Treatment
Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
Mature (8weeks) and immature BMMCs
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Apr 22 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Cat Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Small intestine
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Dog Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
Yes - 0,3% Triton X
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Perfusion fixed with paraformaldehyde and picric acid
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

1-10 of 50 Abreviews or Q&A

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