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Rabbit polyclonal to MAST4
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residue 1900 and 1950 of Human MAST4 (NP_055998.1).
Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 266 kDa.
Use at 5-10 µg/mg of lysate.
Highly expressed in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Microtubule associated serine/threonine protein kinase member 4 antibody
Microtubule-associated serine/threonine-protein kinase 4 antibody
Western blot - Anti-MAST4 antibody (ab87734)
All lanes : Anti-MAST4 antibody (ab87734) at 0.1 µg/ml Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Developed using the ECL technique. Predicted band size: 266 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-MAST4 antibody (ab87734)
Detection of MAST4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87734 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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