Overview

  • Product name
    Anti-MASTL antibody [RIPLY 74C]
    See all MASTL primary antibodies
  • Description
    Mouse monoclonal [RIPLY 74C] to MASTL
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-879 of Human MASTL with a 26kDa proprietary tag.

  • Positive control
    • Human and Mouse MASTL-transfected HEK cell lysates; Raji, Ramos, Dana and MT2 cell lysates; 293T and HeLa transfected cell lysates; Human tonsil, testicle, bone marrow, Hodgkin and Burkitt lymphoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab166647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 97 kDa.
IHC-P 1/60. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
  • Involvement in disease
    Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2700091H24Rik antibody
    • C88295 antibody
    • FLJ14813 antibody
    • GREATWALL antibody
    • Greatwall protein kinase antibody
    • GW antibody
    • GWL antibody
    • GWL_HUMAN antibody
    • hGWL antibody
    • MAST-L antibody
    • Mastl antibody
    • MGC117975 antibody
    • Microtubule associated serine/threonine kinase like antibody
    • Microtubule-associated serine/threonine-protein kinase-like antibody
    • RP11 85G18.2 antibody
    • Serine/threonine-protein kinase greatwall antibody
    • THC2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MASTL with ab166647 at 1/15 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human testicle tissue labeling MASTL with ab166647 at 1/15 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling MASTL with ab166647 at 1/15 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling MASTL with ab166647 at 1/15 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human Burkitt lymphoma tissue labeling MASTL with ab166647 at 1/15 dilution.
  • All lanes : Anti-MASTL antibody [RIPLY 74C] (ab166647) at 1/50 dilution

    Lane 1 : Human MASTL transfected HEK cell lysate
    Lane 2 : Mouse MASTL transfected HEK cell lysate
    Lane 3 : FLT4 transfected HEK cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : Ramos cell lysate
    Lane 6 : Dana cell lysate
    Lane 7 : MT2 cell lysate
    Lane 8 : Human tonsil lysate
    Lane 9 : Human testis lysate

    Predicted band size: 97 kDa

  • All lanes : Anti-MASTL antibody [RIPLY 74C] (ab166647) at 1/50 dilution

    Lane 1 : Mock transfected 293T cell lysate
    Lane 2 : V5-mouse MASTL transfected 293T cell lysate
    Lane 3 : shCtrl transfected HeLa cell lysate
    Lane 4 : shMASTL transfected HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 97 kDa

References

ab166647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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