Overview

  • Product nameAnti-MAT tag antibody [MAT1-87]
  • Description
    Mouse monoclonal [MAT1-87] to MAT tag
  • Specificityab50292 recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ELISAmore details
  • Immunogen

    A synthetic MAT peptide

    HNHRHKHGGGC

    conjugated to KLH.

  • Positive control
    • Cell extracts expressing a C-terminal MAT-tagged protein.
  • General notes


    Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified immunoglobulin
  • Primary antibody notesMonoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.
  • ClonalityMonoclonal
  • Clone numberMAT1-87
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 1 kDa.
IP Use a concentration of 5 - 10 µg/ml.

5 - 10 µg can immunoprecipitate a recombinant C-terminal MAT-tagged protein.

ELISA Use at an assay dependent concentration.

Target

  • RelevanceThe MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors.

References for Anti-MAT tag antibody [MAT1-87] (ab50292)

ab50292 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"