Anti-MATH1/HATH1 antibody (ab85513)

Overview

  • Product nameAnti-MATH1/HATH1 antibody
    See all MATH1/HATH1 primary antibodies
  • Description
    Rabbit polyclonal to MATH1/HATH1
  • Tested applicationsSuitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 288-337 (QLDALHFSTF EDSALTAMMA QKNLSPSLPG SILQPVQEEN SKTSPRSHRS) of Human MATH1/HATH1, NP_005163.

  • Positive control
    • Placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionActivates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATH1 antibody
    • ATOH1 antibody
    • ATOH1_HUMAN antibody
    • Atonal homolog 1 (Drosophila) antibody
    • Atonal homolog 1 antibody
    • bHLHa14 antibody
    • Class A basic helix-loop-helix protein 14 antibody
    • hATH1 antibody
    • Helix loop helix protein hATH 1 antibody
    • Helix-loop-helix protein hATH-1 antibody
    • MATH 1 antibody
    • MATH1 antibody
    • Protein atonal homolog 1 antibody
    see all

Anti-MATH1/HATH1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling MATH1/HATH1 with ab85513.

  • Anti-MATH1/HATH1 antibody (ab85513) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%
  • ab85513 staining MATH1/HATH1 in murine cochlea tissue by Immunohistochemistry.Tissue was fixed in 4% paraformaldehyde, blocked with 2.5% NGS/ 0.5% Triton X-100 for 1 hour and then incubated with ab85513 at a 1/500 dilution for 24 hours at 4°C. The Alexa-Fluor 633 conjugated secondary was used at a 1/500 dilution. Top image: SG spiral ganglion, OC organ of corti. Middle image: CHCs crista hair cells and the organ of corti. Bottom image: HC hair cells.

References for Anti-MATH1/HATH1 antibody (ab85513)

ab85513 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We currently offer three anti-Atoh1 primary antibodies in our catalog. Two of these are also polyclonal, ab75354 and ab85513. We currently do not offer these products in a biotinylated form.

Our cata...

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Thank you for contacting us. We have 2 antibodies ab75354 and ab85513 in catalogues that are anti MATH1/ HATH1. These products are however not tested in IHC with mouse tissue sections. IHC is an untested application so these antibodies a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"