Recombinant fusion protein of Mouse MATH1
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab27667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC (PFA fixed)||1/1000. Mouse embryos were fixed in 4% PFA in phosphate buffer for 2 h at 4 degrees. Rinsed well with phosphate buffer including an overnight rinse (all at 4 degrees). Sunk in 30% sucrose/phos buffer and embedded in OCT for cryosection. Inconclusive results obtained by WB (please see abreview)|
|ICC/IF||Use at an assay dependent concentration.|
ab27667 staining mouse adrenal tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking. The primary antibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.