Our Abpromise guarantee covers the use of ab85824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Activates E box-dependent transcription in collaboration with TCF3/E47. May be a trans-acting factor involved in the development and maintenance of the mammalian nervous system. Transactivates the promoter of its own gene.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Atoh 2 antibody
    • Atoh2 antibody
    • Atonal homolog 2 antibody
    • Atonal protein homolog 2 antibody
    • Atonal protein homolog2 antibody
    • bHLH a2 antibody
    • bHLHa 2 antibody
    • bHLHa2 antibody
    • Class A basic helix-loop-helix protein 2 antibody
    • Helix loop helix protein mATH 2 antibody
    • Helix loop helix protein mATH2 antibody
    • MATH 2 antibody
    • Math2 antibody
    • NDF 6 antibody
    • NDF6 antibody
    • NDF6_HUMAN antibody
    • Neuro D6 antibody
    • NeuroD6 antibody
    • Neurogenic differentiation 6 antibody
    • Neurogenic differentiation factor 6 antibody
    • Nex 1 antibody
    • Nex 1m antibody
    • Nex antibody
    • Nex1 antibody
    • Nex1 m antibody
    • Nex1m antibody
    • Protein atonal homolog 2 antibody
    • Protein atonal homolog2 antibody
    see all

Anti-MATH2/NEUROD6 antibody images

  • All lanes : Anti-MATH2/NEUROD6 antibody (ab85824) at 1 µg/ml

    Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : Brain (Rat) Tissue Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466)
    Lane 6 : U373 MG (Human glioblastoma-astrocytoma, epithelial-like cell line) Nuclear Lysate (ab14902)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab85824 stained PC12 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85824 at 5ug/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti- Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MATH2/NEUROD6 antibody (ab85824)

This product has been referenced in:
  • Klisch TJ  et al. In vivo Atoh1 targetome reveals how a proneural transcription factor regulates cerebellar development. Proc Natl Acad Sci U S A 108:3288-93 (2011). ICC/IF ; Mouse . Read more (PubMed: 21300888) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab85824.
Please use the links above to contact us or submit feedback about this product.


Sign up