Anti-Matriptase 2 antibody (ab28287)
Key features and details
- Rabbit polyclonal to Matriptase 2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Matriptase 2 antibody
See all Matriptase 2 primary antibodies -
Description
Rabbit polyclonal to Matriptase 2 -
Host species
Rabbit -
Specificity
ab28287 recognises the stem region of Matriptase 2. It does not react with the free enzyme. It does not recognise Matriptase 1. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to the stem region of human Matriptase 2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter. -
Tissue specificity
Liver specific. -
Involvement in disease
Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain. -
Domain
Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s). -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 164656 Human
- Omim: 609862 Human
- SwissProt: Q8IU80 Human
- Unigene: 370885 Human
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Alternative names
- IRIDA antibody
- Matriptase-2 antibody
- Membrane bound mosaic serine proteinase matriptase 2 antibody
see all
Images
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ICC/IF image of ab28287 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28287, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab28287 has been referenced in 2 publications.
- Béliveau F et al. Discovery and Development of TMPRSS6 Inhibitors Modulating Hepcidin Levels in Human Hepatocytes. Cell Chem Biol N/A:N/A (2019). PubMed: 31543462
- Gnana-Prakasam JP et al. Retinal expression of the serine protease matriptase-2 (Tmprss6) and its role in retinal iron homeostasis. Mol Vis 20:561-74 (2014). Mouse . PubMed: 24791141