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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Anti-Matriptase 2 antibody - Catalytic domain
See all Matriptase 2 products (9) ...
Description
Rabbit polyclonal to Matriptase 2 - Catalytic domain
Specificity
Reacts with Matriptase 2, and does not recognize matriptase 1.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide based on the catalytic domain of Human Matriptase 2 (within the range aa 760 - 802). (Note: the exact amino acid sequence is proprietary). (Peptide available as ab44743.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
The free catalytic domain is also detected by this antibody.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/1000 - 1/5000.(1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Detects a band of approximately 95 kDa (secreted Matriptase 2) under reducing conditions (predicted molecular weight: 89 kDa).)
Target
Function
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.
Tissue specificity
Liver specific.
Involvement in disease
Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Domain
Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s).
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ8IU80
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matriptase-2 antibody
- Membrane type serine proteinase 2 antibody
- MTSP 2 antibody
see all
Database links
- Entrez Gene: 164656 Human
- Entrez Gene: 71753 Mouse
- Entrez Gene: 315388 Rat
- Omim: 609862 Human
- SwissProt: Q8IU80 Human
- SwissProt: Q6PF94 Mouse
- SwissProt: Q9DBI0 Mouse
- Unigene: 370885 Human
see all
Anti-Matriptase 2 antibody - Catalytic domain images:
Western blot - Matriptase 2 antibody - Catalytic domain (ab42463)
Anti-Matriptase 2 antibody - Catalytic domain (ab42463) at 1/1000 dilution + Human Milk at 1/20 dilution
Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Catalytic domain(ab42463)
ICC/IF image of ab42463 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42463, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Matriptase 2 antibody - Catalytic domain (ab42463)
ab42463 has not yet been referenced specifically in any publications.
Publishing research using ab42463? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"