Matriptase 2 antibody - Catalytic domain (ab42463)

Product Name 

Matriptase 2 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Matriptase 2 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of Human Matriptase 2 (within the range aa 760 - 802).
(Note: the exact amino acid sequence is proprietary).(Peptide available as ab44743.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Reacts with Matriptase 2, and does not recognize matriptase 1.

We have a range of domain specific antibodies for this target. For a full list please see all Matriptase 2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 - 1/5000. 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Detects a band of approximately 95 kDa (secreted Matriptase 2) under reducing conditions (predicted molecular weight: 89 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane (single pass type II membrane protein) and secreted.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes

Relevance 

Matriptase 2 is a type II serine proteinase. Matriptase 2 is an S1A family proteinase, in the PA clan of serine proteinases, which includes trypsin, chymotrypsin, acrosin, and many other key enzymes. A literature alias exists for MTSP2, the Mouse Serine Type Proteinase, granzyme A. Matriptase 2 is organized by an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a stem region and then two CUB domains, three LDL receptor like motifs, and a carboxyterminal catalytic domain. Like matriptase 1, matriptase 2 is shed from the membrane by a cleavage in the stem region, and the catalytic domain can be cleaved from the regulatory domains to generate a free trypsin like enzyme. Matriptase 2 may be more limited in distribution than matriptase 1, and has been identified in the liver by message, but appears to be in kidney, liver and uterus, and in a number of cell lines.

The free catalytic domain is also detected by this antibody.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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