Anti-Matriptase 2 antibody - Catalytic domain (ab56182)
Key features and details
- Rabbit polyclonal to Matriptase 2 - Catalytic domain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Matriptase 2 antibody - Catalytic domain
See all Matriptase 2 primary antibodies -
Description
Rabbit polyclonal to Matriptase 2 - Catalytic domain -
Host species
Rabbit -
Specificity
ab56182 reacts with human Matripase 2 (TMPRSS6), and does not react with the other TMPRSSs. ab56182 recognizes all but the 2 truncated forms of human Matripase 2. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide based on the catalytic domain of Matriptase 2 (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 2.9% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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Note: Glycosylation and other posttranslational modifications make matriptase 2 run at higher than expected mass on reduced SDS PAGE gels.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter. -
Tissue specificity
Liver specific. -
Involvement in disease
Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain. -
Domain
Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s). -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 164656 Human
- Omim: 609862 Human
- SwissProt: Q8IU80 Human
- Unigene: 370885 Human
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Alternative names
- IRIDA antibody
- Matriptase-2 antibody
- Membrane bound mosaic serine proteinase matriptase 2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab56182 has been referenced in 3 publications.
- Krijt J et al. Matriptase-2 and Hemojuvelin in Hepcidin Regulation: In Vivo Immunoblot Studies in Mask Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33800732
- Gurieva I et al. Erythropoietin administration increases splenic erythroferrone protein content and liver TMPRSS6 protein content in rats. Blood Cells Mol Dis 64:1-7 (2017). WB ; Rat . PubMed: 28282554
- Frýdlová J et al. Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats. PLoS One 11:e0148540 (2016). WB . PubMed: 26845567