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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Anti-Matriptase 2 antibody - Cytoplasmic domain
See all Matriptase 2 products (9) ...
Description
Rabbit polyclonal to Matriptase 2 - Cytoplasmic domain
Specificity
ab28286 recognises the cytoplasmic domain of Matriptase 2. It does not recognize Matriptase 1. It also does not recognise the liberated catalytic domain of Matripase 2.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to the cytoplasmic domain of human Matriptase 2.
(Peptide available as ab41266.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
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Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 89 kDa.(EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.)
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.
Tissue specificity
Liver specific.
Involvement in disease
Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Domain
Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s).
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ8IU80
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matriptase-2 antibody
- Membrane type serine proteinase 2 antibody
- MTSP 2 antibody
see all
Database links
- Entrez Gene: 164656 Human
- Entrez Gene: 71753 Mouse
- Entrez Gene: 315388 Rat
- Omim: 609862 Human
- SwissProt: Q8IU80 Human
- SwissProt: Q6PF94 Mouse
- SwissProt: Q9DBI0 Mouse
- Unigene: 370885 Human
see all
Anti-Matriptase 2 antibody - Cytoplasmic domain images:
Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)
ICC/IF image of ab28286 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Matriptase 2 antibody - Cytoplasmic domain (ab28286)
ab28286 has not yet been referenced specifically in any publications.
Publishing research using ab28286? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"