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Anti-Matriptase 2 antibody - Cytoplasmic domain (ab28286)

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Overview

Product name

Anti-Matriptase 2 antibody - Cytoplasmic domain
See all Matriptase 2 products (9) ...

Description

Rabbit polyclonal to Matriptase 2 - Cytoplasmic domain

Specificity

ab28286 recognises the cytoplasmic domain of Matriptase 2. It does not recognize Matriptase 1. It also does not recognise the liberated catalytic domain of Matripase 2.

Tested applications

WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to the cytoplasmic domain of human Matriptase 2.

(Peptide available as ab41266.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: 1/1000 - 1/5000.Pred...Read more →

    WB: 1/1000 - 1/5000.Predicted molecular weight: 89 kDa.(EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.)

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 1 µg/ml

Target

Function

Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.

Tissue specificity

Liver specific.

Involvement in disease

Defects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.

Sequence similarities

Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 3 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.

Domain

Cytoplasmic domain mediates HAMP suppression via proximal promoter element(s).

Cellular localization

Cell membrane.

Target information above from: UniProt accessionQ8IU80 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Matriptase-2 antibody
  • Membrane type serine proteinase 2 antibody
  • MTSP 2 antibody
  • MTSP2 antibody
  • PVAE354 antibody
  • TMPRSS 6 antibody
  • TMPRSS6 antibody
  • TMPS6_HUMAN antibody
  • TMSP 6 antibody
  • TMSP6 antibody
  • Transmembrane protease serine 6 antibody
  • Type II Membrane Serine Proteinase 6 antibody
see all

Anti-Matriptase 2 antibody - Cytoplasmic domain images:

  Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)

Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)

ICC/IF image of ab28286 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Matriptase 2 antibody - Cytoplasmic domain (ab28286)

ab28286 has not yet been referenced specifically in any publications.

Publishing research using ab28286? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"