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Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)
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Product Name
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Matriptase 2 antibody - Cytoplasmic domain
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See all Matriptase 2 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Matriptase 2 - Cytoplasmic domain
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Immunogen
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Synthetic peptide corresponding to the cytoplasmic domain of human Matriptase 2.
(Peptide available as ab41266.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab28286 recognises the cytoplasmic domain of Matriptase 2. It does not recognize Matriptase 1. It also does not recognise the liberated catalytic domain of Matripase 2.
We have a range of domain specific antibodies for this target. For a full list please see all Matriptase 2 antibodies
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weights (Human): 89 kDa when membrane bound and 95kDa for the secreted (glycosilated) form (on reduced SDS PAGE gels). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane (single pass type II membrane protein) and secreted.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
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Relevance
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Matriptase 2 is a type II serine proteinase. Matriptase 2 is an S1A family proteinase, in the PA clan of serine proteinases, which includes trypsin, chymotrypsin, acrosin, and many other key enzymes. A literature alias exists for MTSP2, the Mouse Serine Type Proteinase, granzyme A. Matriptase 2 is organized by an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a stem region and then two CUB domains, three LDL receptor like motifs, and a carboxyterminal catalytic domain. Like matriptase 1, matriptase 2 is shed from the membrane by a cleavage in the stem region, and the catalytic domain can be cleaved from the regulatory domains to generate a free trypsin like enzyme. Matriptase 2 may be more limited in distribution than matriptase 1, and has been identified in the liver by message, but appears to be in kidney, liver and uterus, and in a number of cell lines.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Matriptase 2 antibody - Cytoplasmic domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Matriptase 2 antibody - Cytoplasmic domain images, references, products related to ab28286 and other tools.
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Matriptase 2 antibody - Cytoplasmic domain images:
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 Immunocytochemistry/ Immunofluorescence-Matriptase 2 antibody - Cytoplasmic domain(ab28286)
ICC/IF image of ab28286 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to Matriptase 2
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