Anti-Matriptase 2 antibody - Cytoplasmic domain (ab56180)


  • Product nameAnti-Matriptase 2 antibody - Cytoplasmic domain
    See all Matriptase 2 primary antibodies
  • Description
    Rabbit polyclonal to Matriptase 2 - Cytoplasmic domain
  • Specificityab56180 reacts with Matripase 2 (TMPRSS6) and does not react with the other TMPRSSs. ab56180 recognizes all of the listed forms of Matripase 2.
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the aminoterminal end (cytoplasmic domain) of Matripase 2 (Human).



Our Abpromise guarantee covers the use of ab56180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 89 kDa.

    Note: Glycosylation and other posttranslational modifications make Matripase 2 run at higher than expected mass on reduced SDS PAGE gels.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Required to sense iron deficiency. Overexpression suppresses activation of the HAMP promoter.
    • Tissue specificityLiver specific.
    • Involvement in diseaseDefects in TMPRSS6 are the cause of iron-refractory iron deficiency anemia (IRIDA) [MIM:206200]; also known as hypochromic microcytic anemia with defect in iron metabolism or hereditary iron-handling disorder or pseudo-iron-deficiency anemia. Key features include congenital hypochromic microcytic anemia, very low mean corpuscular erythrocyte volume, low transferrin saturation, abnormal iron absorption characterized by no hematologic improvement following treatment with oral iron, and abnormal iron utilization characterized by a sluggish, incomplete response to parenteral iron.
    • Sequence similaritiesBelongs to the peptidase S1 family.
      Contains 2 CUB domains.
      Contains 3 LDL-receptor class A domains.
      Contains 1 peptidase S1 domain.
    • DomainCytoplasmic domain mediates HAMP suppression via proximal promoter element(s).
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • IRIDA antibody
      • Matriptase-2 antibody
      • Membrane bound mosaic serine proteinase matriptase 2 antibody
      • Membrane type serine proteinase 2 antibody
      • MTSP 2 antibody
      • MTSP2 antibody
      • PVAE354 antibody
      • TMPRSS 6 antibody
      • TMPRSS6 antibody
      • TMPS6_HUMAN antibody
      • TMSP 6 antibody
      • TMSP6 antibody
      • Transmembrane protease serine 6 antibody
      • Type II Membrane Serine Proteinase 6 antibody
      see all

    Anti-Matriptase 2 antibody - Cytoplasmic domain images

    • All lanes : Anti-Matriptase 2 antibody - Cytoplasmic domain (ab56180) at 1/1000 dilution

      Lane 1 : Cell media from human embryonic kidney HEK293 cells
      Lane 2 : Cell media from human embryonic kidney HEK293 cells
      Lane 3 : Cell media from human embryonic kidney HEK293 cells

      Predicted band size : 89 kDa

    References for Anti-Matriptase 2 antibody - Cytoplasmic domain (ab56180)

    This product has been referenced in:
    • Foka P  et al. A complex signaling network involving protein kinase CK2 is required for hepatitis C virus core protein-mediated modulation of the iron-regulatory hepcidin gene expression. Cell Mol Life Sci N/A:N/A (2014). WB . Read more (PubMed: 24718935) »
    • Lakhal S  et al. Regulation of Type II Transmembrane Serine Proteinase TMPRSS6 by Hypoxia-inducible Factors: NEW LINK BETWEEN HYPOXIA SIGNALING AND IRON HOMEOSTASIS. J Biol Chem 286:4090-7 (2011). WB ; Human . Read more (PubMed: 20966077) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (Hepatoma cells)
    Specification Hepatoma cells
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C

    Dr. Alexios Dimitriadis

    Verified customer

    Submitted Jul 15 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (293T transfected with Hu matriptase 2.FLAG)
    Total protein in input 1e+007 cells
    Specification 293T transfected with Hu matriptase 2.FLAG
    Immuno-precipitation step Protein A

    Ms. Schimanski Lisa

    Verified customer

    Submitted Oct 22 2008