Matriptase 2 antibody - Stem region (ab28287)

Product Name 

Matriptase 2 antibody - Stem region

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Matriptase 2 - Stem region

Immunogen 

Synthetic peptide corresponding to the stem region of human Matriptase 2.

(Peptide available as ab41267.)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab28287 recognises the stem region of Matriptase 2. It does not react with the free enzyme. It does not recognise Matriptase 1.

We have a range of domain specific antibodies for this target. For a full list please see all Matriptase 2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 89 kDa. Secreted Matriptase 2 is glycosylated and runs at approximately 95 kDa on reduced SDS PAGE gels. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane (single pass type II membrane protein) and secreted.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> TMPRSS
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes

Relevance 

Matriptase 2 is a type II serine proteinase. Matriptase 2 is an S1A family proteinase, in the PA clan of serine proteinases, which includes trypsin, chymotrypsin, acrosin, and many other key enzymes. A literature alias exists for MTSP2, the Mouse Serine Type Proteinase, granzyme A. Matriptase 2 is organized by an aminoterminal cytoplasmic domain, followed by a transmembrane domain, a stem region and then two CUB domains, three LDL receptor like motifs, and a carboxyterminal catalytic domain. Like matriptase 1, matriptase 2 is shed from the membrane by a cleavage in the stem region, and the catalytic domain can be cleaved from the regulatory domains to generate a free trypsin like enzyme. Matriptase 2 may be more limited in distribution than matriptase 1, and has been identified in the liver by message, but appears to be in kidney, liver and uterus, and in a number of cell lines.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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