Overview

  • Product nameAnti-Matrix protein 1 antibody
    See all Matrix protein 1 primary antibodies
  • Description
    Goat polyclonal to Matrix protein 1
  • SpecificityPurified matrix protein (M). Recognizes the matrix protein for any strain of Influenza A. Conservation of the matrix protein sequence between hemagluttinin/Neuraminidase typed strains. Does not react with HEp-2 cells by indirect immunofluorescence.
  • Tested applicationsSuitable for: ICC/IF, ELISA, Conjugation, WBmore details
    Unsuitable for: IHC
  • Species reactivity
    Does not react with Influenza B, Adenovirus, Respiratory syncytial virus and Parainfluenza viruses(1-3). Not yet tested in other species.
  • Immunogen

    corresponding to Matrix protein 1. Purified M1 protein, Influenza A-Phillipines (H3N2).

Properties

Applications

Our Abpromise guarantee covers the use of ab20910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Conjugation Use at an assay dependent dilution.
WB 1/500. Detects a band of approximately 27,54 kDa (predicted molecular weight: 27,54 kDa).
  • Application notesIs unsuitable for IHC.
  • Target

    • RelevanceInfluenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.
    • Cellular localizationCytoplasmic
    • Alternative names
      • M1 antibody
      • Matrix Protein 1 antibody
      • Matrix Protein I antibody
      • Membrane matrix protein M1 antibody

    Anti-Matrix protein 1 antibody images

    • Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.

      See Abreview

    • All lanes : Anti-Matrix protein 1 antibody (ab20910) at 1/500 dilution

      Lane 1 : 293T cells transfected with GFP-M1 and infected with influenza A virus
      Lane 2 : 293T cells transfected with GFP-M1 and mock infected
      Lane 3 : 293T cells transfected with pcDNA-NP and infected with influenza A virus
      Lane 4 : 293T cells transfected with pcDNA-NP and mock infected
      Lane 5 : 293T cells infected with influenza A virus
      Lane 6 : 293T cells mock infected
      Lane 7 : M1 expressed using a minireplicon system
      Lane 8 : Minireplicon system control (no PB2)

      Secondary
      Donkey anti-goat (IRDye® 800CW) at 1/10000 dilution

      Predicted band size : 27,54 kDa
      Observed band size : 27,54 kDa

      This image is courtesy of an abreview submitted by Maria Amorim.

      See Abreview

    References for Anti-Matrix protein 1 antibody (ab20910)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (kidney, epithelial cells)
    Specification kidney, epithelial cells
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
    Fixative Formaldehyde
    Username

    Dr. Maria Amorim

    Verified customer

    Submitted Apr 03 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (kidney, epithelial cells)
    Loading amount 100000 cells
    Specification kidney, epithelial cells
    Treatment transfected (or not) with GFP-M1 or pcDNA-NP or a minireplicon system and infected (or not) with influenza A virus
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
    Username

    Dr. Maria Amorim

    Verified customer

    Submitted Mar 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"