Anti-MAVS antibody (ab25084)

  Immunocytochemistry/ Immunofluorescence - MAVS antibody (ab25084)

ICC/IF image of ab25084 stained human HEK293 cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab25084, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  Western blot - MAVS antibody (ab25084)

All lanes : Anti-MAVS antibody (ab25084) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 57 kDa
Observed band size : 57,70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

The bands at 70 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAVS antibody - ChIP Grade (ab25084)

IHC image of MAVS staining in human liver carcinoma FFPE section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25084, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.