Overview

  • Product nameAnti-MAX antibody [73C5a] - ChIP Grade
    See all MAX primary antibodies
  • Description
    Mouse monoclonal [73C5a] to MAX - ChIP Grade
  • Tested applicationsSuitable for: Flow Cyt, ChIP, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human MAX

Properties

Applications

Our Abpromise guarantee covers the use of ab53570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 20797623
WB Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
Dot blot Use at an assay dependent concentration.

Target

  • RelevanceMAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • bHLHd4 antibody
    • bHLHd5 antibody
    • bHLHd6 antibody
    • bHLHd7 antibody
    • bHLHd8 antibody
    • Helix loop helix zipper protein antibody
    • MAX protein antibody
    • MGC10775 antibody
    • MGC11225 antibody
    • MGC18164 antibody
    • MGC34679 antibody
    • MGC36767 antibody
    • MYC associated factor X antibody
    • Myc binding novel HLH/LZ protein antibody
    • Orf 1 antibody
    • Orf1 antibody
    • Protein max antibody
    see all

Anti-MAX antibody [73C5a] - ChIP Grade images

  • Anti-MAX antibody [73C5a] - ChIP Grade (ab53570) + immunised recombinant protein

    Predicted band size : 18 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-MAX antibody [73C5a] - ChIP Grade (ab53570)

This product has been referenced in:
  • Wang M  et al. Common genetic variation in ETV6 is associated with colorectal cancer susceptibility. Nat Commun 7:11478 (2016). ChIP ; Human . Read more (PubMed: 27145994) »
  • Xue G  et al. c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2a and promotes tumor angiogenesis and metastasis by upregulating FGF2. Oncogene N/A:N/A (2014). Read more (PubMed: 24704828) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab53570.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"