Overview

  • Product nameAnti-MAX antibody
    See all MAX primary antibodies
  • Description
    Rabbit polyclonal to MAX
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residue 110 and 160 of Human MAX (NP_002373.3).

  • Positive control
    • HeLa and 293T whole cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal prostate

Properties

Applications

Our Abpromise guarantee covers the use of ab101271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceMAX is a basic helix-loop-helix, leucine zipper protein that forms heterodimers with MYC and MAD. It is involved in regulating gene expression.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • bHLHd4 antibody
    • bHLHd5 antibody
    • bHLHd6 antibody
    • bHLHd7 antibody
    • bHLHd8 antibody
    • Helix loop helix zipper protein antibody
    • MAX protein antibody
    • MGC10775 antibody
    • MGC11225 antibody
    • MGC18164 antibody
    • MGC34679 antibody
    • MGC36767 antibody
    • MYC associated factor X antibody
    • Myc binding novel HLH/LZ protein antibody
    • Orf 1 antibody
    • Orf1 antibody
    • Protein max antibody
    see all

Anti-MAX antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAX with ab101271 at 1/1000 (1µg/ml).
  • IHC image of MAX staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101271, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-MAX antibody (ab101271) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 18 kDa


    Exposure time : 3 minutes
  • Detection of MAX in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101271 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents Immunoprecipitates from control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-MAX antibody (ab101271)

ab101271 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"