Anti-Maxi Potassium channel beta antibody (ab3587)

Overview

  • Product nameAnti-Maxi Potassium channel beta antibody
    See all Maxi Potassium channel beta primary antibodies
  • Description
    Rabbit polyclonal to Maxi Potassium channel beta
  • Specificityab3587 is specific for the Beta-1 subunit of Maxi K+. It does not detect the beta-2 subunit
  • Tested applicationsSuitable for: IHC-P, WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human Maxi Potassium channel beta aa 90-103.
    Sequence:

    LYHTEDTRDQNQQC


    (Peptide available as ab5023)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesPotassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel beta peptide (ab5023). Detects an ~28 kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts.
ICC Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 16116057

Target

  • RelevanceMaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits.
  • Cellular localizationMembrane; multi-pass membrane protein
  • Database links
  • Alternative names
    • BK channel beta subunit antibody
    • BK channel subunit beta 1 antibody
    • BKbeta antibody
    • BKbeta1 antibody
    • Calcium activated potassium channel subfamily M subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta antibody
    • Charybdotoxin receptor subunit beta 1 antibody
    • Hbeta1 antibody
    • hslo beta antibody
    • K(VCA)beta 1 antibody
    • K(VCA)beta antibody
    • KCNMB 1 antibody
    • KCNMB1 antibody
    • Large conductance Ca2+ activated K+ channel beta 1 subunit antibody
    • Maxi K channel beta subunit antibody
    • Maxi K channel subunit beta 1 antibody
    • Potassium large conductance calcium activated channel subfamily M beta member 1 antibody
    • Slo beta 1 antibody
    • SLO beta antibody
    see all

Anti-Maxi Potassium channel beta antibody images

References for Anti-Maxi Potassium channel beta antibody (ab3587)

This product has been referenced in:
  • Leo MD  et al. Dynamic regulation of ß1 subunit trafficking controls vascular contractility. Proc Natl Acad Sci U S A 111:2361-6 (2014). Read more (PubMed: 24464482) »
  • Nourian Z  et al. Large Conductance Ca2+-Activated K+ Channel (BKCa) a-Subunit Splice Variants in Resistance Arteries from Rat Cerebral and Skeletal Muscle Vasculature. PLoS One 9:e98863 (2014). WB ; Rat . Read more (PubMed: 24921651) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.
We do have the blocking peptide available as ab5023.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (Cartilage, articular)
Specification Cartilage, articular
Fixative Neutral buffered formalin
Antigen retrieval step None
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Horse Cell lysate - whole cell (Chondrocytes isolated from equine cartilage)
Loading amount 25 µg
Specification Chondrocytes isolated from equine cartilage
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chicken Tissue lysate - other (cerebellum)
Loading amount 22 µg
Specification cerebellum
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Username

Dr. Soledad Miranda-Rottmann

Verified customer

Submitted Jul 25 2006

Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that ab3587 is specific for the Beta-1 subunit of Maxi K+. Further to a sequence comparison between the immunogen and the sequence of the beta-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"