Anti-Maxi Potassium channel beta antibody (ab3587)

Overview

  • Product name
    Anti-Maxi Potassium channel beta antibody
    See all Maxi Potassium channel beta primary antibodies
  • Description
    Rabbit polyclonal to Maxi Potassium channel beta
  • Specificity
    ab3587 is specific for the Beta-1 subunit of Maxi K+. It does not detect the beta-2 subunit
  • Tested applications
    Suitable for: IHC-P, WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Dog, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human Maxi Potassium channel beta aa 90-103.
    Sequence:

    LYHTEDTRDQNQQC


    (Peptide available as ab5023)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel beta peptide (ab5023). Detects an ~28 kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts.
ICC Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 16116057

Target

  • Relevance
    MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits.
  • Cellular localization
    Membrane; multi-pass membrane protein
  • Database links
  • Alternative names
    • BK channel beta subunit antibody
    • BK channel subunit beta 1 antibody
    • BKbeta antibody
    • BKbeta1 antibody
    • Calcium activated potassium channel subfamily M subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta 1 antibody
    • Calcium activated potassium channel subunit beta antibody
    • Charybdotoxin receptor subunit beta 1 antibody
    • Hbeta1 antibody
    • hslo beta antibody
    • K(VCA)beta 1 antibody
    • K(VCA)beta antibody
    • KCNMB 1 antibody
    • KCNMB1 antibody
    • Large conductance Ca2+ activated K+ channel beta 1 subunit antibody
    • Maxi K channel beta subunit antibody
    • Maxi K channel subunit beta 1 antibody
    • Potassium large conductance calcium activated channel subfamily M beta member 1 antibody
    • Slo beta 1 antibody
    • SLO beta antibody
    see all

Images

References

This product has been referenced in:
  • Nystoriak MA  et al. Ser1928 phosphorylation by PKA stimulates the L-type Ca2+ channel CaV1.2 and vasoconstriction during acute hyperglycemia and diabetes. Sci Signal 10:N/A (2017). Read more (PubMed: 28119464) »
  • Wang Y  et al. The Involvement of Ca(2+) Signal Pathways in Distal Colonic Myocytes in a Rat Model of Dextran Sulfate Sodium-induced Colitis. Chin Med J (Engl) 129:1185-92 (2016). WB . Read more (PubMed: 27174327) »

See all 13 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.
We do have the blocking peptide available as ab5023.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cartilage, articular)
Specification
Cartilage, articular
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Horse Cell lysate - whole cell (Chondrocytes isolated from equine cartilage)
Loading amount
25 µg
Specification
Chondrocytes isolated from equine cartilage
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

Submitted Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chicken Tissue lysate - other (cerebellum)
Loading amount
22 µg
Specification
cerebellum
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Username

Dr. Soledad Miranda-Rottmann

Verified customer

Submitted Jul 25 2006

Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that ab3587 is specific for the Beta-1 subunit of Maxi K+. Further to a sequence comparison between the immunogen and the sequence of the beta-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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