Overview

  • Product nameAnti-MAZ antibody
    See all MAZ primary antibodies
  • Description
    Rabbit polyclonal to MAZ
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide within residues: FPVFPCTLLA PPFPVLGLDS RGVGGLMNSF PPPQGHAQNP LQVGAELQSR , corresponding to N terminal amino acids 2-51 of Human MAZ (NP_002374)

  • Positive control
    • 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83397 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use at an assay dependent concentration. PubMed: 22415301
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
  • Tissue specificityPresent in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
  • Sequence similaritiesContains 6 C2H2-type zinc fingers.
  • Cellular localizationNucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAZ antibody
    • MAZ_HUMAN antibody
    • MAZI antibody
    • MYC associated zinc finger protein antibody
    • Myc-associated zinc finger protein antibody
    • Pur-1 antibody
    • Pur1 antibody
    • Purine binding transcription factor antibody
    • Purine-binding transcription factor antibody
    • SAF 1 antibody
    • SAF 2 antibody
    • SAF-1 antibody
    • SAF-2 antibody
    • SAF-3 antibody
    • Serum amyloid A activating factor 2 antibody
    • Transcription factor Zif87 antibody
    • ZF87 antibody
    • Zif87 antibody
    • Zinc finger protein 801 antibody
    • ZNF801 antibody
    see all

Anti-MAZ antibody images

  • Anti-MAZ antibody (ab83397) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling MAZ with ab83397 at 10µg/ml.

References for Anti-MAZ antibody (ab83397)

This product has been referenced in:
  • Smits M  et al. Myc-associated zinc finger protein (MAZ) is regulated by miR-125b and mediates VEGF-induced angiogenesis in glioblastoma. FASEB J 26:2639-47 (2012). IHC-P ; Human . Read more (PubMed: 22415301) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab83397.
As the datasheet indicates the concentration of this antibody is 1 mg/ml and the unit size is 50 µg.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"