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Synthetic peptide within residues: FPVFPCTLLA PPFPVLGLDS RGVGGLMNSF PPPQGHAQNP LQVGAELQSR , corresponding to N terminal amino acids 2-51 of Human MAZ (NP_002374)
Our Abpromise guarantee covers the use of ab83397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
|IHC-P||Use at an assay dependent concentration. PubMed: 22415301|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling MAZ with ab83397 at 10µg/ml.