Overview

  • Product nameAnti-MBD1 antibody [100B272.1.] - ChIP Grade
    See all MBD1 primary antibodies
  • Description
    Mouse monoclonal [100B272.1.] to MBD1 - ChIP Grade
  • Specificityab45028 detects MBD1, isoform 1.
  • Tested applicationsWB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (

    SESEDGAGSPPPYRR

    ) of human MBD1.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ChIP
  • Application notesChIP: Use at an assay dependent dilution (PMID 15327775).
    WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesContains 3 CXXC-type zinc fingers.
      Contains 1 MBD (methyl-CpG-binding) domain.
    • DomainThe methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
    • Post-translational
      modifications
      Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • CXXC 3 antibody
      • CXXC-type zinc finger protein 3 antibody
      • CXXC3 antibody
      • MBD 1 antibody
      • MBD1 antibody
      • MBD1 antibody
      • MBD1_HUMAN antibody
      • MECP1 COMPLEX antibody
      • Methyl CpG binding domain protein 1 antibody
      • Methyl CpG binding domain protein 1 antibody
      • Methyl CpG binding domain protein 1 isoform PCM1 antibody
      • Methyl CpG binding protein MBD1 antibody
      • Methyl CpG binding protein splice variant 1 antibody
      • Methyl CpG binding protein splice variant 2 antibody
      • Methyl CpG binding protein splice variant 3 antibody
      • Methyl CpG binding protein splice variant 4 antibody
      • Methyl-CpG-binding domain protein 1 antibody
      • Methyl-CpG-binding protein MBD1 antibody
      • PCM 1 antibody
      • PCM 1 antibody
      • PCM1 antibody
      • Protein containing methyl CpG binding domain 1 antibody
      • Protein containing methyl-CpG-binding domain 1 antibody
      • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
      • RFT antibody
      • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
      see all

    Anti-MBD1 antibody [100B272.1.] - ChIP Grade images

    • Lane 1 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 2 µg/ml
      Lane 2 : Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028) at 1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate


      Predicted band size : 67 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    References for Anti-MBD1 antibody [100B272.1.] - ChIP Grade (ab45028)

    This product has been referenced in:
    • Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell 15:595-605 (2004). ChIP ; Human . Read more (PubMed: 15327775) »
    • Reese BE  et al. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol 23:3226-36 (2003). Read more (PubMed: 12697822) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"