Overview

  • Product name
    Anti-MBD1 antibody [7A3C12A9]
    See all MBD1 primary antibodies
  • Description
    Mouse monoclonal [7A3C12A9] to MBD1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse MBD1 aa 401-635.
    Sequence:

    KRLLPSAGSG SGEGAGLRPY QTHQTHQKRP ASARQLQLSS PLKAPWAVVT APPGPVRDSR KQQAGRGSVL PQPDTDFVFL QEGTSSAMQM PGTAAASTEV PVQAAQCSAP SWVVALPQVK QETADAPEEW TAVTTFLTSS TLQSGFPSKA ADPDLSPVKQ EPPGPEEDGE EKKDDVSETT PAEEIGGVGT PVITEIFSLG GTRLRDAEAW LPRLHKLLAV NEKEYFTELQ LKEEV


    Database link: Q9Z2E2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Clonality
    Monoclonal
  • Clone number
    7A3C12A9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 67 kDa.

Target

  • Function
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Domain
    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • Post-translational
    modifications
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CXXC 3 antibody
    • CXXC-type zinc finger protein 3 antibody
    • CXXC3 antibody
    • MBD 1 antibody
    • Mbd1 antibody
    • MBD1_HUMAN antibody
    • MECP1 COMPLEX antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 isoform PCM1 antibody
    • Methyl CpG binding protein MBD1 antibody
    • Methyl CpG binding protein splice variant 1 antibody
    • Methyl CpG binding protein splice variant 2 antibody
    • Methyl CpG binding protein splice variant 3 antibody
    • Methyl CpG binding protein splice variant 4 antibody
    • Methyl-CpG-binding domain protein 1 antibody
    • Methyl-CpG-binding protein MBD1 antibody
    • PCM 1 antibody
    • PCM1 antibody
    • Protein containing methyl-CpG-binding domain 1 antibody
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
    • RFT antibody
    • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
    see all

References

ab187734 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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