• Product nameAnti-MBD1 antibody
    See all MBD1 primary antibodies
  • Description
    Mouse monoclonal to MBD1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey, Orangutan
  • Immunogen

    Recombinant fragment: HHLGPTLKPT LATRTAQPDH TQAPTKQEAG GGFVLPPPGT DLVFLREGAS SPVQVPGPVA ASTEALLQEA QCSGLSWVVA LPQVKQEKAD TQDE, corresponding to amino acids 415-508 of Human MBD1 (NP_056671), with a 26 kDa proprietary tag.

  • Positive control
    • Partial tagged recombinant Human MBD1 protein (Immunogen), IMR-32 cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab89534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ELISA Use at an assay dependent dilution.


  • FunctionTranscriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • DomainThe methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • Post-translational
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CXXC 3 antibody
    • CXXC-type zinc finger protein 3 antibody
    • CXXC3 antibody
    • MBD 1 antibody
    • Mbd1 antibody
    • MBD1_HUMAN antibody
    • MECP1 COMPLEX antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 isoform PCM1 antibody
    • Methyl CpG binding protein MBD1 antibody
    • Methyl CpG binding protein splice variant 1 antibody
    • Methyl CpG binding protein splice variant 2 antibody
    • Methyl CpG binding protein splice variant 3 antibody
    • Methyl CpG binding protein splice variant 4 antibody
    • Methyl-CpG-binding domain protein 1 antibody
    • Methyl-CpG-binding protein MBD1 antibody
    • PCM 1 antibody
    • PCM1 antibody
    • Protein containing methyl-CpG-binding domain 1 antibody
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
    • RFT antibody
    • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
    see all

Anti-MBD1 antibody images

References for Anti-MBD1 antibody (ab89534)

ab89534 has not yet been referenced specifically in any publications.

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