Overview

  • Product name
    Anti-MBD1 antibody - ChIP Grade
    See all MBD1 primary antibodies
  • Description
    Rabbit polyclonal to MBD1 - ChIP Grade
  • Specificity
    This antibody detects human methyl CpG binding domain protein 1 (MBD1) recombinant protein.
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MBD1 aa 593-605.
    Sequence:

    RSKDLKKPGARKQ


    (Peptide available as ab4918)

Properties

Applications

Our Abpromise guarantee covers the use of ab2846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with MBD1 peptide (ab4918).
ChIP 1/50.

Target

  • Function
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Domain
    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • Post-translational
    modifications
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CXXC 3 antibody
    • CXXC-type zinc finger protein 3 antibody
    • CXXC3 antibody
    • MBD 1 antibody
    • Mbd1 antibody
    • MBD1_HUMAN antibody
    • MECP1 COMPLEX antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 isoform PCM1 antibody
    • Methyl CpG binding protein MBD1 antibody
    • Methyl CpG binding protein splice variant 1 antibody
    • Methyl CpG binding protein splice variant 2 antibody
    • Methyl CpG binding protein splice variant 3 antibody
    • Methyl CpG binding protein splice variant 4 antibody
    • Methyl-CpG-binding domain protein 1 antibody
    • Methyl-CpG-binding protein MBD1 antibody
    • PCM 1 antibody
    • PCM1 antibody
    • Protein containing methyl-CpG-binding domain 1 antibody
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
    • RFT antibody
    • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
    see all

Images



  • Predicted band size : 83 kDa
    Western blot showing approx 83 kD band, representing recombinant human MBD 1

References

This product has been referenced in:
  • Laget S  et al. MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress. Epigenetics 9:546-56 (2014). Read more (PubMed: 24434851) »
  • Katryniok C  et al. Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity. Biochim Biophys Acta 1801:49-57 (2010). WB, ChIP ; Human . Read more (PubMed: 19781662) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (Breast Cancer Cell Line MCF-7)
Specification
Breast Cancer Cell Line MCF-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Amplification of sonicated genomic DNA (gDNA) with gene-specific primers prior immunoprecipitation served as positive control.
Negative control
DNA immunoprecipitated with the antibody IgG served as negative control
Username

Dr. Heidi Schwarzenbach

Verified customer

Submitted May 01 2007

Thank you for your enquiry and for your patience. I have been looking into the issue that you have raised. Both ab3753 and ab2846 are originated outside Abcam and by two different sources. The originator of ab2846 said that all evidence that th...

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