Overview

  • Product nameAnti-MBD1 antibody - ChIP GradeSee all MBD1 primary antibodies ...
  • Description
    Rabbit polyclonal to MBD1 - ChIP Grade
  • SpecificityThis antibody detects human methyl CpG binding domain protein 1 (MBD1) recombinant protein.
  • Tested applicationsWB, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MBD1 aa 593-605.
    Sequence:

    RSKDLKKPGARKQ


    (Peptide available as ab4918)

Properties

Applications

Our Abpromise guarantee covers the use of ab2846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with MBD1 peptide (ab4918).
ChIP 1/50.

Target

  • FunctionTranscriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • DomainThe methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • Post-translational
    modifications
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CXXC 3 antibody
    • CXXC-type zinc finger protein 3 antibody
    • CXXC3 antibody
    • MBD 1 antibody
    • MBD1 antibody
    • MBD1_HUMAN antibody
    • MECP1 COMPLEX antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 isoform PCM1 antibody
    • Methyl CpG binding protein MBD1 antibody
    • Methyl CpG binding protein splice variant 1 antibody
    • Methyl CpG binding protein splice variant 2 antibody
    • Methyl CpG binding protein splice variant 3 antibody
    • Methyl CpG binding protein splice variant 4 antibody
    • Methyl-CpG-binding domain protein 1 antibody
    • Methyl-CpG-binding protein MBD1 antibody
    • PCM 1 antibody
    • PCM 1 antibody
    • PCM1 antibody
    • Protein containing methyl CpG binding domain 1 antibody
    • Protein containing methyl-CpG-binding domain 1 antibody
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
    • RFT antibody
    • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
    see all

Anti-MBD1 antibody - ChIP Grade images



  • Predicted band size : 83 kDa
    Western blot showing approx 83 kD band, representing recombinant human MBD 1

References for Anti-MBD1 antibody - ChIP Grade (ab2846)

This product has been referenced in:
  • Katryniok C  et al. Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity. Biochim Biophys Acta 1801:49-57 (2010). WB, ChIP ; Human . Read more (PubMed: 19781662) »
  • Müller I  et al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer 10:297 (2010). WB ; Mouse . Read more (PubMed: 20565761) »

See all 3 Publications for this product

Product Wall

Application ChIP
Sample Human Cell lysate - nuclear (Breast Cancer Cell Line MCF-7)
Specification Breast Cancer Cell Line MCF-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control Amplification of sonicated genomic DNA (gDNA) with gene-specific primers prior immunoprecipitation served as positive control.
Negative control DNA immunoprecipitated with the antibody IgG served as negative control
Username

Dr. Heidi Schwarzenbach

Verified customer

Submitted May 01 2007

Thank you for your enquiry and for your patience. I have been looking into the issue that you have raised. Both ab3753 and ab2846 are originated outside Abcam and by two different sources. The originator of ab2846 said that all evidence that th...

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