Overview

  • Product name
  • Description
    Goat polyclonal to MBD2
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    C-RNDPLNQNKGKPDLN

    , corresponding to internal sequence amino acids 226-240 of Human MBD2

  • Positive control
    • Human Adrenal, Colon and Skin tissue; Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/64000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 µg/ml. Predicted molecular weight: 43 kDa.

Target

  • Function
    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • Tissue specificity
    Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Images

  • ab105323, at 2.5 µg/ml, staining MBD2 in formalin-fixed, paraffin-embedded Human Adrenal tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab105323, at 2.5 µg/ml, staining MBD2 in formalin-fixed, paraffin-embedded Human Colon tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab105323, at 2.5 µg/ml, staining MBD2 in formalin-fixed, paraffin-embedded Human Skin tissue by Immunohistochemistry, using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-MBD2 antibody (ab105323) at 0.3 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 43 kDa

References

ab105323 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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