Overview

  • Product name
  • Description
    Rabbit polyclonal to MBD2
  • Specificity
    This antibody is specific for MBD2 (N term). Rat - Abreview feedback has stated that this antibody works on rat prostate. It gives some non-specific band in rat testis, probably due to presence of other MBD isoforms.
  • Tested applications
    Suitable for: ICC/IF, IP, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MBD2 aa 120-151 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9UBB5

  • Positive control
    • A375 cell lysate and mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IP Use at an assay dependent concentration.
ELISA 1/1000.
WB 1/100 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • Tissue specificity
    Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Anti-MBD2 antibody images

  • ab38646 immunoprecipitating MBD2 in human K562 whole cell lysate. 15µg of cell lysate was incubated with primary antibody (2 µg/ml in RIPA buffer) and matrix (Protein A/G) for 12 hours at 4°C. For western blotting a HRP-conjugated goat anti-rabbit IgG polyclonal (1/2500) was used as the secondary antibody.

    See Abreview

  • ab38646 staining MBD2 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in TBS) for 12 hours at 4°C. An undiluted alkaline phosphatase-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-MBD2 antibody (ab38646) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab38646 staining MBD2 in rat liver cultured cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 10 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/400 dilution.

References for Anti-MBD2 antibody (ab38646)

This product has been referenced in:
  • Raciti GA  et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep 7:43526 (2017). ChIP ; Mouse . Read more (PubMed: 28266632) »
  • Qin T  et al. Epigenetic synergy between decitabine and platinum derivatives. Clin Epigenetics 7:97 (2015). ChIP ; Human . Read more (PubMed: 26366234) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Cell (MEL)
Specification
MEL
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 13 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (K562)
Specification
K562
Total protein in input
15 µg
Username

Abcam user community

Verified customer

Submitted Apr 16 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample
Human Tissue sections (normal tonsil)
Specification
normal tonsil
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Cell lysate - whole cell (MEL, 293T cell)
Specification
MEL, 293T cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Min Young Kim

Verified customer

Submitted Jun 25 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Rat Cell lysate - nuclear (Rat Neuronal Cortex)
Specification
Rat Neuronal Cortex
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step
Semiquantitative PCR
Positive control
Mecp2 antibody.
Negative control
purified rabbit IgG
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Verified customer

Submitted Nov 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Liver, cultured cell line)
Specification
Liver, cultured cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 06 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Rat Cell lysate - whole cell (Liver)
Specification
Liver
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (1% final concent
Detection step
Real-time PCR
Positive control
anti-acetyl Histone H3
Negative control
normal rabbit IgG
Username

Abcam user community

Verified customer

Submitted Nov 12 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Prostate (ventrale lobe))
Loading amount
5 µg
Specification
Prostate (ventrale lobe)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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