Overview

  • Product nameAnti-MBD2 antibody
    See all MBD2 primary antibodies
  • Description
    Goat polyclonal to MBD2
  • SpecificityExpected to recognize isoform 1 only.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    RNDPLNQNKGKPDLN

    , corresponding to internal sequence amino acids 226-240 of Human MBD2

  • Positive control
    • Jurkat cell lysate Human colon

Properties

Applications

Our Abpromise guarantee covers the use of ab58241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • FunctionBinds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • Tissue specificityHighly expressed in brain, heart, kidney, stomach, testis and placenta.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • Mbd2 antibody
    • MBD2_HUMAN antibody
    • MBD2a antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl-CpG-binding domain protein 2 antibody
    • Methyl-CpG-binding protein MBD2 antibody
    • NY CO 41 antibody
    see all

Anti-MBD2 antibody images

  • Anti-MBD2 antibody (ab58241) at 0.3 µg/ml + Jurkat cell lysate at 35 µg

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Detected by chemiluminescence.
  • ab58241 at 3.5 µg/ml staining MBD2 in Human colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.

References for Anti-MBD2 antibody (ab58241)

This product has been referenced in:
  • Müller I  et al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer 10:297 (2010). WB ; Mouse . Read more (PubMed: 20565761) »
  • Mastroeni D  et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 31:2025-37 (2010). IHC-Fr ; Human . Read more (PubMed: 19117641) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Xenopus egg extract)
Specification Xenopus egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
Username

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Verified customer

Submitted Mar 08 2011

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"