The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
Use a concentration of 2 - 4 µg/ml.
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Highly expressed in brain, heart, kidney, stomach, testis and placenta.
Contains 1 MBD (methyl-CpG-binding) domain.
Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
ab58241 at 3.5 µg/ml staining MBD2 in Human colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed by AP-staining.
Müller I et al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer10:297 (2010).
Read more (PubMed: 20565761) »
Mastroeni D et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging31:2025-37 (2010).
Read more (PubMed: 19117641) »