Overview

  • Product name
  • Description
    Rabbit polyclonal to MBD2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Guinea pig, Pig, Chimpanzee, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 100 and 150 of human MBD2 (NP_003918.1)

  • Positive control
    • Human Breast Carcinoma, Stomach Adenocarcinoma and Testicular Seminoma tissues; Mouse Teratoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab84753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
    • Tissue specificity
      Highly expressed in brain, heart, kidney, stomach, testis and placenta.
    • Sequence similarities
      Contains 1 MBD (methyl-CpG-binding) domain.
    • Cellular localization
      Nucleus. Nuclear, in discrete foci. Detected at replication foci in late S phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Demethylase antibody
      • DMTase antibody
      • MBD 2 antibody
      • Mbd2 antibody
      • MBD2_HUMAN antibody
      • MBD2a antibody
      • Methyl CpG binding domain protein 2 antibody
      • Methyl CpG binding protein MBD2 antibody
      • Methyl-CpG-binding domain protein 2 antibody
      • Methyl-CpG-binding protein MBD2 antibody
      • NY CO 41 antibody
      see all

    Images

    • ab84753 at 1/100 dilution staining MBD2 in human breast carcinoma by Immunohistochemistry/Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1:100.
    • ab84753 at 1/250 dilution staining MBD2 in human stomach carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84753 at 1/250 dilution staining MBD2 in human testicular seminoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84753 at 1/250 dilution staining MBD2 in mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References

    ab84753 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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