MBD2 (methyl-CpG binding domain protein 2) belongs to a family of MBD domain containing proteins. This family of proteins associates with heterochromatin by their interaction with methylated DNA at CpG islands, and appear to 'read' and 'translate' the DNA methylation signal into transcriptional repression by recruiting silencing complexes and histone deacetylases among other proteins. MBD2 is the methyl-binding component of the MeCP1 complex, which allows MBD2 to target HDAC/chromatin remodeling activity to methylated templates.
MBD2 Binding Activity Quantification Assay Kit (ab113473) allows the user to measure binding activity of MBD2 to methylated DNA in human cells/tissues. It can also be used to determine inhibitors of MBD2 activity.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 12ml||1 x 25ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Affinity Antibody, 100 µg/mL||1 x 25µl||1 x 50µl|
|Assay Buffer||1 x 1.8ml||1 x 4ml|
|Detection Antibody, 200 µg/mL||1 x 10µl||1 x 20µl|
|Developing Solution||1 x 6ml||1 x 12ml|
|Methylated DNA, 25 µg/mL||1 x 50µl||1 x 100µl|
|Stop Solution||1 x 3ml||1 x 6ml|
Our Abpromise guarantee covers the use of ab113473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113473 has not yet been referenced specifically in any publications.