Anti-MBD2 + MBD3 antibody (ab3757)

Overview

  • Product nameAnti-MBD2 + MBD3 antibody
    See all MBD2 + MBD3 primary antibodies
  • Description
    Rabbit polyclonal to MBD2 + MBD3
  • SpecificityThis antibody recognises the two Drosophila MBD 2 + 3 variants.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Recombinant full length protein (Drosophilia melanogaster).

  • General notes

     

    Methyl CpG binding domain proteins (MBDs) are a family of mammalian proteins that specifically bind methylated regions of DNA. There are two methyl CpG binding complexes, MeCP1 and MeCP2. MeCP2 is comprised of a single protein also denoted MeCP2. MeCP1 is composed of four different proteins termed MBD 1 (also known as PCM 1), MBD 2, MBD 3, and MBD 4 (also known as MED 1).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferserum with 0.02% sodium azide
  • PurityWhole antiserum
  • Primary antibody notesMethyl CpG binding domain proteins (MBDs) are a family of mammalian proteins that specifically bind methylated regions of DNA. There are two methyl CpG binding complexes, MeCP1 and MeCP2. MeCP2 is comprised of a single protein also denoted MeCP2. MeCP1 is composed of four different proteins termed MBD 1 (also known as PCM 1), MBD 2, MBD 3, and MBD 4 (also known as MED 1).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 25,35 kDa (predicted molecular weight: 25,35 kDa).

Detects two bands of approximately 25 kDa and 35 kDa which correspond to the predicted molecular weights of recombinant drosophila MBD 2 + 3 short and long forms, respectively.

Target

  • RelevanceDNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
  • Cellular localizationNuclear
  • Alternative names
    • Demethylase antibody
    • DMTase antibody
    • MBD 2 antibody
    • MBD 3 antibody
    • Methyl CpG binding domain protein 2 antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD2 antibody
    • Methyl CpG binding protein MBD3 antibody
    see all

Anti-MBD2 + MBD3 antibody images



  • Predicted band size : 25,35 kDa


    Rabbit polyclonal to Drosophila MBD 2 + 3 (at a 1/500 dilution):

    1. Recombinant drosophila MBD 2 + 3 (long form)
    2. Recombinant drosophila MBD 2 + 3d (short form)
    3. Nuclear extract of Drosophila embryos where the two drosophila MBD 2 + 3 forms are detected.

References for Anti-MBD2 + MBD3 antibody (ab3757)

ab3757 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us for technical support and filling in our online protocol questionnaire, it is very useful to understand you problem. MBD2 is part of a methyl DNA binding complex. These things are often quite difficult to ChIP. The upsta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"