Overview

  • Product name
  • Description
    Rabbit polyclonal to MBD3
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, OrangutanDoes not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 265 - 291 (

    ACAEDDDEED EEEEEEEPDP DPEMEHV

    ) of Human MBD3 (NP_003917.1).

  • Positive control
    • HeLa or 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI181826 antibody
    • AU019209 antibody
    • MBD 3 antibody
    • Mbd3 antibody
    • MBD3: methyl CpG binding domain protein 3 antibody
    • MBD3_HUMAN antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD3 antibody
    • Methyl-CpG-binding domain protein 3 antibody
    • Methyl-CpG-binding protein MBD3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 33 kDa


    Exposure time : 30 seconds
  • SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458.
    20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2).
    Detection: chemoluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Cell lysate - nuclear (ES cells)
Specification
ES cells
Total protein in input
1000 µg
Username

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Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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