Overview

  • Product nameAnti-MBD3 antibody
    See all MBD3 primary antibodies
  • Description
    Rabbit polyclonal to MBD3
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, OrangutanDoes not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 265 - 291 (

    ACAEDDDEED EEEEEEEPDP DPEMEHV

    ) of Human MBD3 (NP_003917.1).

  • Positive control
    • HeLa or 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab91458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionDoes not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI181826 antibody
    • AU019209 antibody
    • MBD 3 antibody
    • Mbd3 antibody
    • MBD3: methyl CpG binding domain protein 3 antibody
    • MBD3_HUMAN antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD3 antibody
    • Methyl-CpG-binding domain protein 3 antibody
    • Methyl-CpG-binding protein MBD3 antibody
    see all

Anti-MBD3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 33 kDa


    Exposure time : 30 seconds
  • SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458.
    20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2).
    Detection: chemoluminescence with an exposure time of 10 seconds.

References for Anti-MBD3 antibody (ab91458)

This product has been referenced in:
  • Shimbo T  et al. MBD3 localizes at promoters, gene bodies and enhancers of active genes. PLoS Genet 9:e1004028 (2013). ChIP ; Human . Read more (PubMed: 24385926) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Cell lysate - nuclear (ES cells)
Specification ES cells
Total protein in input 1000 µg
Username

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Verified customer

Submitted Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"