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Rabbit polyclonal to MBD3
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Does not react with:
Synthetic peptide corresponding to a region within C terminal amino acids 265 - 291 (
ACAEDDDEED EEEEEEEPDP DPEMEHV
) of Human MBD3 (NP_003917.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
Use at 2-10 µg/mg of lysate.
Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
Contains 1 MBD (methyl-CpG-binding) domain.
Nucleus. Nuclear, in discrete foci.
Information by UniProt
MBD 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD3 antibody (ab91458)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MBD3 with ab91458 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-MBD3 antibody (ab91458)
All lanes : Anti-MBD3 antibody (ab91458) at 0.1 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 33 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-MBD3 antibody (ab91458)
SSA1 was immunoprecipitated from 1mg HeLa whole cell lysate using 10µg ab91458.
20% of the immunoprecipitate was loaded per lane, and probed with ab91458 at 1µg/ml (lane 1) or a control IgG (lane2). Detection: chemoluminescence with an exposure time of 10 seconds.
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