Overview

  • Product name
    Anti-MBD3 antibody - Carboxyterminal end
    See all MBD3 primary antibodies
  • Description
    Rabbit polyclonal to MBD3 - Carboxyterminal end
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of human MBD3.

  • Positive control
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and transfected/ non-transfected 293 cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus. Nuclear, in discrete foci.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI181826 antibody
    • AU019209 antibody
    • MBD 3 antibody
    • Mbd3 antibody
    • MBD3: methyl CpG binding domain protein 3 antibody
    • MBD3_HUMAN antibody
    • Methyl CpG binding domain protein 3 antibody
    • Methyl CpG binding protein MBD3 antibody
    • Methyl-CpG-binding domain protein 3 antibody
    • Methyl-CpG-binding protein MBD3 antibody
    see all

Images

  • All lanes : Anti-MBD3 antibody - Carboxyterminal end (ab71777) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : MBD3 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71777 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

References

This product has been referenced in:
  • Hata K  et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Read more (PubMed: 24336734) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab71777.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up