Validated using a knockout cell line
Recombinant
RabMAb

Anti-MBD3 antibody [EPR9912] (ab155105)

Overview

  • Product name
    Anti-MBD3 antibody [EPR9912]
    See all MBD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR9912] to MBD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MBD3. The exact sequence is proprietary.

  • Positive control
    • F9, C6, HeLa, 293T and Jurkat cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR9912
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MBD3 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: F9 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab155105 observed at 33 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab155105 was shown to recognize MBD3 when MBD3 knockout samples were used, along with additional cross-reactive bands. Wild-type and MBD3 knockout samples were subjected to SDS-PAGE. ab155105 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-MBD3 antibody [EPR9912] (ab155105) at 1/5000 dilution (purified) + F9 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MBD3 antibody [EPR9912] (ab155105) at 1/5000 dilution (purified)

      Lane 1 : HEK293 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MBD3 antibody [EPR9912] (ab155105) at 1/1000 dilution (unpurified)

      Lane 1 : F9 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 33 kDa

    References

    ab155105 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155105.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up