MBD3 overexpression 293T lysate (whole cell) (ab94293)

Overview

  • Product name
    MBD3 overexpression 293T lysate (whole cell)
  • Description
    MBD3 overexpression lysate
  • General notes
    ab94293 is a 293T cell transfected lysate in which Human MBD3 has been transiently over-expressed using a pCMV-MBD3 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Similarity: Contains 1 MBD (methyl-CpG-binding) domain.

Applications

Our Abpromise guarantee covers the use of ab94293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94293 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-MBD3 antibody (ab89440) at 1/500 dilution

    Lane 1 : MBD3 overexpression 293T lysate (whole cell) (ab94293)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94293 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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