• Product name
  • Description
    Rabbit polyclonal to MBD4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide corresponding to amino acids 541 to 554 of mouse MBD4 conjugated to KLH via an N terminal added cysteine residue. The sequence is conserved in human.

  • Positive control
    • Nuclear extracts of K562 cell line.
  • General notes
    Storage in frost free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified using the immunizing peptide immobilized on agarose.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab12187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa). Staining of the MBD4 band in immunoblotting is specifically inhibited by the immunizing peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
    • Sequence similarities
      Contains 1 MBD (methyl-CpG-binding) domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 N(4) ethenocytosine glycosylase antibody
      • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
      • G/T mismatch glycosylase antibody
      • G/U mismatch glycosylase antibody
      • MBD 4 antibody
      • MBD4 antibody
      • MBD4_HUMAN antibody
      • MED 1 antibody
      • MED1 antibody
      • Methyl CpG binding domain protein 4 antibody
      • Methyl CpG binding endonuclease 1 antibody
      • Methyl CpG binding protein MBD4 antibody
      • Methyl-CpG-binding domain protein 4 antibody
      • Methyl-CpG-binding endonuclease 1 antibody
      • Methyl-CpG-binding protein MBD4 antibody
      • Mismatch specific DNA N glycosylase antibody
      • Mismatch-specific DNA N-glycosylase antibody
      • Putative methyl CpG binding protein antibody
      see all

    Anti-MBD4 antibody images

    • Lane 1 : Anti-MBD4 antibody (ab12187) at 1 µg/ml (Rabbit anti-MBD4)
      Lane 2 : Anti-MBD4 antibody (ab12187) at 0.5 µg/ml (Rabbit anti-MBD4)

      Lane 1 : K-562 cells
      Lane 2 : K-562 cells

      Goat anti-rabbit IgG, peroxidase and a chemiluminescent substrate

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    References for Anti-MBD4 antibody (ab12187)

    This product has been referenced in:
    • Pettersen HS  et al. UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21745813) »
    • Bertoni C  et al. Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides. Nucleic Acids Res 37:7468-82 (2009). WB ; Mouse . Read more (PubMed: 19854937) »

    See all 3 Publications for this product

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