Overview

  • Product name
  • Description
    Rabbit polyclonal to MBD4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 68-300 of Human MBD4 (BC011752).

  • Positive control
    • K562, MCF7, Human fetal liver and Human fetal heart lysates

Properties

  • Form
    Lyophilised:Reconstitute in 200µl sterile H2O.
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

Target

  • Function
    Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similarities
    Contains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Images

  • All lanes : Anti-MBD4 antibody (ab124460) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate


    Predicted band size : 66 kDa

References

ab124460 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab124460.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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