Overview

  • Product nameAnti-MBD4 antibody
    See all MBD4 primary antibodies
  • Description
    Rabbit polyclonal to MBD4
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 1 and 50 of Human MBD4 (NP_003916.1)

  • Positive control
    • Human Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma and Testicular Seminoma tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab84754 was affinity purified using an epitope specific to MBD4 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab84754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining.

Target

  • FunctionMismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
  • Sequence similaritiesContains 1 MBD (methyl-CpG-binding) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 N(4) ethenocytosine glycosylase antibody
    • G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody
    • G/T mismatch glycosylase antibody
    • G/U mismatch glycosylase antibody
    • MBD 4 antibody
    • MBD4 antibody
    • MBD4_HUMAN antibody
    • MED 1 antibody
    • MED1 antibody
    • Methyl CpG binding domain protein 4 antibody
    • Methyl CpG binding endonuclease 1 antibody
    • Methyl CpG binding protein MBD4 antibody
    • Methyl-CpG-binding domain protein 4 antibody
    • Methyl-CpG-binding endonuclease 1 antibody
    • Methyl-CpG-binding protein MBD4 antibody
    • Mismatch specific DNA N glycosylase antibody
    • Mismatch-specific DNA N-glycosylase antibody
    • Putative methyl CpG binding protein antibody
    see all

Anti-MBD4 antibody images

  • ab84754 at 1/250 dilution staining MBD4 in Human testicular seminoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab84754 at 1/250 dilution staining MBD4 in Human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

References for Anti-MBD4 antibody (ab84754)

This product has been referenced in:
  • Hata K  et al. DNA methylation and methyl-binding proteins control differential gene expression in distinct cortical areas of macaque monkey. J Neurosci 33:19704-14 (2013). Macaque Monkey . Read more (PubMed: 24336734) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"