The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionBinds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro).
Tissue specificityDetected in heart, placenta, liver, skeletal muscle, kidney and pancreas.
Involvement in diseaseDefects in MBD5 are the cause of mental retardation autosomal dominant type 1 (MRD1) [MIM:156200]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.