Overview

  • Product name
  • Description
    Rabbit polyclonal to MBNL2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 46-95 (ENGRVIACFD SLKGRCSREN CKYLHPPTHL KTQLEINGRN NLIQQKTAAA) of Human MBNL2.

  • Positive control
    • Human fetal brain lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3'UTR of ITGA3. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. May play a role in myotonic dystrophy pathophysiology (DM).
  • Tissue specificity
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the muscleblind family.
    Contains 4 C3H1-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. Greater concentration in the nucleus. Expressed in or near large cytoplasmic adhesion plaques (PubMed:16273094). Location in the cytoplasm is microtubule-dependent (PubMed:16273094). In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts (PubMed:11929853).
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781H1296 antibody
    • MBLL antibody
    • MBLL39 antibody
    • Mbnl2 antibody
    • MBNL2_HUMAN antibody
    • MGC120625 antibody
    • MGC120626 antibody
    • MGC120628 antibody
    • Muscleblind like 2 antibody
    • muscleblind like protein 1 antibody
    • muscleblind like protein 2 antibody
    • muscleblind like protein like 39 antibody
    • muscleblind like splicing regulator 2 antibody
    • Muscleblind-like protein 1 antibody
    • Muscleblind-like protein 2 antibody
    • Muscleblind-like protein-like 39 antibody
    • Muscleblind-like protein-like antibody
    • OTTHUMP00000178763 antibody
    • PRO2032 antibody
    • RP11-128N14.1 antibody
    see all

Images



  • Predicted band size : 41 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling MBNL2 with ab105331 at 1.0µg/ml.


  • Predicted band size : 41 kDa
    Western blot analysis of HepG2 cell lysate labeling MBNL2 with ab105331 at 1.0µg/ml.


  • Predicted band size : 41 kDa
    Western blot analysis of HeLa cell lysate labeling MBNL2 with ab105331 at 1.0µg/ml.
  • Anti-MBNL2 antibody (ab105331) at 0.5 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size : 41 kDa
    Gel concentration: 12%

References

ab105331 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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