Overview

  • Product name
  • Description
    Mouse polyclonal to MBNL3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, human MBNL3 (AAH42090, 258 a.a).

  • Positive control
    • human placenta lysate MBNL3 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36, 29 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.
  • Tissue specificity
    Highly expressed in the placenta.
  • Sequence similarities
    Belongs to the muscleblind family.
    Contains 4 C3H1-type zinc fingers.
  • Cellular localization
    Nucleus. Cytoplasm. Greater concentration in the nucleus. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts.
  • Information by UniProt
  • Database links
  • Alternative names
    • A530038J18Rik antibody
    • AI661274 antibody
    • CHCR antibody
    • Cys3His CCG1 required protein antibody
    • Cys3His CCG1-required protein antibody
    • E430034C16Rik antibody
    • MBLX antibody
    • MBLX39 antibody
    • MBNL 3 antibody
    • Mbnl3 antibody
    • MBNL3_HUMAN antibody
    • MBXL antibody
    • Muscleblind like 3 antibody
    • Muscleblind like protein 3 antibody
    • Muscleblind like splicing regulator 3 antibody
    • Muscleblind like X linked protein antibody
    • Muscleblind-like protein 3 antibody
    • Muscleblind-like X-linked protein antibody
    • OTTMUSP00000019072 antibody
    • OTTMUSP00000019073 antibody
    • OTTMUSP00000019074 antibody
    • Protein HCHCR antibody
    • RP23-98P13.1 antibody
    see all

Images

  • Anti-MBNL3 antibody (ab68759) at 1/500 dilution + human placental lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MBNL3 antibody (ab68759) at 1/500 dilution

    Lane 1 : MBNL3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

References

ab68759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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